Just getting started with waveRAPID? This video is for you! Learn how to use the built-in presets for weak, intermediate, and tight binders, and see how different settings affect your results. Includes real data comparisons and tips for choosing the right injection time and concentration. Fast-track your assay setup with confidence.
#waveRAPID #KineticAnalysis #BindingKinetics #WeakBinders #TightBinders #BiomolecularInteractionAnalysis #LabelFreeDetection #GCItechnology #GratingCoupledInterferometry
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What do confidence intervals really tell you in waveRAPID analysis? In this quick guide, we break down one of the most asked-about QC parameters: the correlation between kinetic estimates like Rmax and on-rate. Learn how to interpret heatmaps, identify parameter identifiability, and spot negative correlations that could affect your fit. A must-watch for anyone analyzing binding data with waveRAPID!
#waveRAPID #KineticAnalysis #BindingKinetics #BiomolecularInteractionAnalysis #LabelFreeDetection #GCItechnology #GratingCoupledInterferometry
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Unlock the full power of waveRAPID in this all-in-one tutorial designed for both beginners and experienced GCI users! Discover how waveRAPID compares to traditional multi-cycle kinetics, and learn to fine-tune your assays by optimizing concentration, injection time, and binding affinity (Rmax). With hands-on examples, software walkthroughs, and clever visualizations—including a fun hedgehog analogy and AI-generated graphs—this video is packed with practical tips to help you get the most out of your kinetic experiments.
#waveRAPID #KineticAssays #BindingKinetics #GCItechnology #LabelFreeDetection
#BiomolecularInteractionAnalysis #SPRanalysis #MultiCycleKinetics #DrugDiscovery #GratingCoupledInterferometry
00:00 – Introduction & Overview
00:16 – GCI Tips & Multi-Cycle Kinetics Warm-Up
01:59 – Troubleshooting Blanks in Double Referencing
02:59 – waveRAPID vs. Traditional Kinetics
04:35 – Interpreting waveRAPID Data: First Example
05:56 – Adjusting Concentration & Injection Time
07:14 – Using waveRAPID Presets for Assay Setup
08:01 – Comparing Binding Profiles Across Settings
10:26 – Understanding Parameter Correlation (QC Tips)
12:28 – FAQ: Why Use Dissociation Data Only?
14:36 – When to Include Association Data
17:48 – Case Study: Optimizing Real Assay Data
21:55 – From waveRAPID to Multi-Cycle Kinetics
22:32 – Final Thoughts & Takeaways
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If you’ve ever wondered how waveRAPID determines on-rates using only dissociation data, this video is for you! We break down one of the most frequently asked questions from new users and explain why dissociation-only fitting works, how on-rate information is embedded in curve shapes, and when it makes sense to include association data. With clear visual examples and practical tips, you’ll gain a deeper understanding of parameter sensitivity and how to get the most accurate results from your kinetic analysis.
#waveRAPID #KineticAnalysis #BindingKinetics #DissociationData #BiomolecularInteractionAnalysis #LabelFreeDetection #GCItechnology #DrugDiscovery #GratingCoupledInterferometry
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Take your assay development to the next level with this hands-on guide to optimizing kinetic experiments using waveRAPID. Learn how to detect weak or tight binding interactions, choose the right settings for your analyte, and interpret complex behaviors like biphasic responses and saturation. This video also shows how to align your waveRAPID results with ITC and traditional multi-cycle kinetics for robust validation. Ideal for researchers working on small molecule–protein interactions or transitioning from SPR to waveRAPID.
#waveRAPID #KineticAnalysis #BindingKinetics #IsothermalTitrationCalorimetry #PEAQ-ITC #BiomolecularInteractionAnalysis #LabelFreeDetection #GCItechnology #SPRanalysis #GratingCoupledInterferometry
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New to waveRAPID? This quick explainer breaks down how waveRAPID differs from traditional multi-cycle kinetics. Learn how it uses repeated analyte pulses from a single concentration to generate high-quality kinetic data—faster and with less prep time. Perfect for scientists looking to streamline their binding studies.
#waveRAPID #KineticAssays #BindingKinetics #GCItechnology #LabelFreeDetection
#BiomolecularInteractionAnalysis #SPRanalysis #MultiCycleKinetics #DrugDiscovery #GratingCoupledInterferometry
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In this interactive walkthrough, we evaluate a real waveRAPID dataset and discuss how to improve it. Learn when to increase concentration, adjust injection time, and how to recognize a strong interaction profile. A great clip for sharpening your kinetic interaction skills.
#waveRAPID #KineticAnalysis #BindingKinetics #BiomolecularInteractionAnalysis #LabelFreeDetection #GCItechnology #GratingCoupledInterferometry
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